DNA standards ranging from 50 pg/mL to 1 μg/mL were detected using the Quant-iT PicoGreen dsDNA Assay Kit and SpectraMax readers (data from the SpectraMax iD5 reader are shown, but other SpectraMax readers gave similar results). application or budget. Specialized filters allow for IR fluorescence detection ranging from 10-40 times moreNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The drawing shows a standard microplate of 15 mm height covered with a lid. See SpectraMax® Microplate Reader User Manual Download Page. collect in lysis buffer, incubate on ice for 2h, isolate RNA & measure. Pick the best speed, sensitivity, and price for your needs. How to properly unlock iD series instrument. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. SpectraMax iD3 - Virtual Demo. your experiments in seconds. Prepare your iD series instrument for shipment. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The absorbance was determined using a SpectraMax iD3 Multi-mode microplate reader (Molecular Devices, USA) with 480 nm excitation wavelength and 516 nm emission wavelength. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. The PathCheck sensor can extend the instrument linearity by a factor of three or more. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Whether you are looking for an intuitive, easy-to-use reader for your busy,. 제조 출고일 : 2019년1월14일. TUNE Tunable Wavelength Detection Cartridge. BMG LABTECH - CLARIOstar Plus . Cell viability는 세포막 Integrity 또는. Explore a wealth of applications with the future-ready SpectraMax i3x. Patented user-exchangeable cartridge design expands the system's detection capability making it. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. NFC-based personalization and enhanced touchscreen workflows provide walk-up usability The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Figure 3. Reader spectramax microplate id3 molecular devices multi mode boundaries functionality instrumentSpectramax i3x multi-mode microplate reader with minimax imaging Molecular devices spectramax i3x 使用される 販売のために 価格. -65. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. By taking preventive maintenance measures, and making sure to go through the troubleshooting tips, you can make sure to extend the. If you are using a SpectraMax ® iD3 or iD5, ensure the Firmware versions are up-to-date. We would like to show you a description here but the site won’t allow us. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. It features a touchscreen interface, a built-in camera, and a patented optical system for optimal performance. . If no sound is heard during initialization, contact technical support. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. • SpectraMax iD3/iD5 Multi-Mode Microplate Readers • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only)The plate was mixed on an orbital shaker for 15 s and immediately read using a SpectraMax iD3 (Molecular Devices; San Jose, CA, USA) and an integration time of 400 ms. Align the two slots on the bottle holder with the two pegs on the rail. Using the SpectraMax iD3 and iD5 readers with the SpectraMax Injector Cartridge with SmartInject Technology, and FLIPR Calcium 6 and 6-QF Assay Kits, we have demonstrated that it is possible to. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. i3. no. Conclusion. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax iD3. 吸光、蛍光、発光測定に対応。. See SpectraMax® Microplate Reader User Manual Download Page. GFP expression imaged on the cytometer. This user guide provides detailed instructions on how to set up, operate, and maintain the SpectraMax iD3 Microplate Reader. SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Table 2. For wavelengths in the UV range above 220 nm,. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. The SpectraMax iD3 has a large, intuitive, high-resolution touchscreen (pictured left) and an embedded software package so users can quickly and easily set up custom protocols as well as take advantage of Molecular Devices preconfigured protocols. Cet appareil ISM est confomre à la norme NMB-001 du Canada. MICROPLATE READERS. 존재하지 않는 이미지입니다. Tutorial video on how to start a plate read on the SpectraMax iD3. All data are in μM and are n ≥ 3 independent experiments. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eSpectramax i3x reader, spectramax id5 reader, spectramax id3 reader, general, filtermax f5 reader,. Event 한정수량! 특별할인가 7500000원 Vat 별도 정가 9000000원 xMark 가격 별도 문의 선택의 기준을 높였다! Microplate Reader 02-3471-6500 042-824-7000…Reader spectramax id3 microplate molecular devices multi mode functionality boundaries expanding laboratory configured fully bring designed instrument. SpectraMax Injector Cartridge with SmartInject™ Technology. Panel 2 demonstrates how a computer in the lab may be used to control the reader using SoftMax Pro. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. HTRF-compatible SpectraMax Multi-Mode Readers. jayesh desale; Status: Reviewer . Learn how to quickly and easily start a plate read using the high-resolution touchscreen interface. 3) At the bottom of the strip, click on "Show. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. With optimized reagents, the option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax® Pro Software, the SpectraMax® iD3 and iD5 Multi-Mode Microplate. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. When the user applies a set temperature of 37°C, the top is heated to 37. One of the most popular methods for assaying cell health is fluorescence detection with a microplate reader. Model : SpectraMax ID3. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. SpectraMax iD3 Multi-Mode Microplate Reader The SpectraMax iD3 reader features a large, high-resolution touchscreen interface with an embedded software package allowing users to set up custom protocols, use pre-loaded protocols, and run experiments without the need for a dedicated computer workstation. With Optimized Reagents And The Industry-leading Data Acquisition And Apr 2th, 2023Application Note For FP Validation For Spectramax ParadigmPlate; Optimize For. book Page 1 Wednesday, July 7, 2010 2:09 PMSpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. #iD3) • AgraQuant Melamine Test Kit (Romer Labs cat. SpectraMax iD3 reader. GM2000 Register or Sign In. NFC-based personalization and enhanced touchscreen workflows provide walk-up usability The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 5. Subscribe to our newsletter. The Zone at 91-3, Victoria, British Columbia. Prepare your i3/i3x instrument for shipment. 2 only) SpectraMax M-series Microplate readers. 4-µL DNA standards from 2 ng/µL to 1000 ng/µL were read in absorbance detection mode with a preconfigured protocol in SoftMaxMolecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. -49. SpectraMax ABS SpectraTest ABS2 v1. SpectraMax M5/M5e/M4/M3/M2/M2e. Learn more about its features, specifications, and applications in this user. It may be named "Pothos". Whether you are looking for an intuitive, easy-to-use reader for your busy,. This five-mode microplate reader with automatic filter identification and western blot capability is the complete laboratory. The screw remains inside the instrument. 305 reference level saturation. S2). 존재하지 않는. Whether you are looking for an intuitive, easy-to-use reader for your busy,. , Ltd. Request a Quote!SpectraMax iD3 Multi-Mode Microplate Reader The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you. The SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. ImageXpress. Tutorial on how to quickly and easily view your results on the SpectraMax iD3 Multi-Mode Microplate Reader. ATP standard curves read on SpectraMax microplate readers. The chamber. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The drawing shows a standard microplate of 15 mm height covered with a lid. 01) between the GFP-transfected and mock-transfected cells at an initial seeding. spr SpectraMax iD3 SpectraTest ABS2 v1. SpectraMaxiD3Multi-ModeMicroplateReaderUserGuide 4 5054747H Read 70 ResultLibrary 71 Chapter6:ReadModesandReadTypes 77 AbsorbanceReadMode 79 FluorescenceIntensityReadMode 83 SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use This process should take about 3 - 5 minutes. 7°C and the bottom to 36. 優れた光学システムにはキセノンフラッシュランプ. 1 mL) at a specific concentration was added to a 96-well microplate and preincubated in a microplate reader (SpectraMax iD3 multimode microplate reader, Molecular Devices, San Jose, CA, USA) for 5 min. In addition, the SpectraMax® iD5 can be expanded to include bottom. 1 mL) to the substrate solution. SoftMax Pro GxP Compliance Software. These errors are seen with SoftMax Pro 5. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). The SpectraMax iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. With optimized reagents, the option of. Use the provided 3. Learn more about its features, specifications, and applications in this user guide. 99 (Figure 2). Step 3. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Fluorescence was measured using a plate reader (Spectra Max iD3, Molecular Devices) set to an exc/em wavelength of 535/590 nm (aggregate) or. iD5. Cancer stem cells (CSC) contribute to chemotherapy resistance, relapse, and metastasis. Enter email and password. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Find regional support. application or budget. Data Sheet-SpectraMax ID3 Multi-Mode Microplate Reader. The SoftMax® Touch Software is installed in. Next, plates were washed twice with dilution buffer solution. 4. Introduction. The SpectraMax iD3 reader is fully compatible with the SpectraDrop™ Micro-Volume Microplate, enabling quantitation of precious low-volume samples. Category 2 and 3 microplate readers use gradient heating as well. Find regional support. Substrate solution (0. Experience the identifiably different SpectraMax iD3 Multi-Mode Microplate Reader. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. 5. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. Superoxide dismutase (SOD) and catalase (CAT) activities were measured using a microplate reader (Spectra Max iD3, Molecular Devices, San Jose, CA, USA) at 450 and 240 nm according to the SOD (M0102A) and CAT (M0103A) kits (Suzhou Michy Biomedical Technology Co. SpectraMax® i3/i3x and iD3/iD5: Preparing instrument for shipment. 313 reference gain check fail. 4-µL DNA standards from 2 ng/µL to 1000 ng/µL were read in absorbance detection mode with a preconfigured protocol in SoftMaxSpectraMax® Paradigm® 多功能检测平台采用专利保护的卡式模块设计，允许用户在两分钟内进行实时添 加、组合、更换检测模块。这种酶标仪模块化理念的变革既保证了各种通用检测应用，又提供了拓展那些不 断增加的日新月异的各种新特殊应用的可能性。Application note demonstrating that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. -54. 3: The cuvette chamber. We would like to show you a description here but the site won’t allow us. 4 µL DNA standards from 2 ng/µL to 1000 ng/µL were read in absorbance detection mode with a preconfigured protocol in SoftMax ProSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Featuring a high-resolution touchscreen, ultra-cooled PMT for increased sensitivity, and NFC reader for a complete personalized workflow. SpectraMax iD3 Multi-Mode Microplate Reader The SpectraMax iD3 reader features a large, high-resolution touchscreen interface with an embedded software package allowing users to set up custom protocols, use pre-loaded protocols, and run experiments without the need for a dedicated computer workstation. Tutorial video on how to view your experiment results on the SpectraMax iD3. Member since: 2019. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostSPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance (ANOVA) (Table 1). Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the How to start a plate read on the SpectraMax iD5 hybrid multi-mode microplate reader 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide FlexStation® 3 Multi-Mode Microplate Reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with. Sunnyvale, California (PRWEB) February 07, 2017 -- Molecular Devices announced today the launch of the new SpectraMax® iD3 Multi-Mode Microplate Reader, a cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. DS_Store","path":"References_and_Manuals. 315 can't find zero order. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Restart the system. Table of Contents. The SpectraMax iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax Gemini EM/XPS. Auction Details HTG Molecular Diagnostics, Inc. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Figure 2-23: Bottle holder To install the bottle holder: 1. application or budget. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. LoadingThis video introduces the Spectramax iD3 Multi-mode microplate reader by Molecular Devices. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. DNA has approximately 23% less absorbance when dissolved in TE-buffered saline rather than water (Figure. The SpectraMax iD3 reader features a large, high-resolution touchscreen interface with an embedded software package allowing users to set up custom protocols, use pre-loaded protocols, and run experiments without the need for a dedicated computer workstation. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors theHow to start a plate read on the SpectraMax iD5 hybrid multi-mode microplate reader0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideFlexStation® 3 Multi-Mode Microplate Reader. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence. Please see exact video and photos below. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD5 reader is your all access. -27. Absorbance was read at a test wavelength of 440 nm and a reference wavelength of 690 nm using a multi-mode microplate reader (Spectra Max iD3; Molecular Devices, United States). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Unplug the power cable from the back of the instrument. The . 2b68. Examples: “Press carefully to engage,” “ Do not. Unparalleled performance on a personalized platform. Shake intensity. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme kinetics. The SpectraMax ID3 plate reader can measure uv-visible absorption, fluorescence, and luminescence using microplate formats from 6- to 384-wells. SpectraMaxQuant AccuBlue Pico dsDNA Assay; SpectraMax Quant AccuBlue HiRange dsDNA Assay; SpectraMax Quant AccuBlue Pico dsDNA (6. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The corrected absorbance is within 5% of the value. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Our scalable ELISA Workcells provide walkaway time, increasing throughput, effectiveness and efficiency of the assay procedure, and reproducibility. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Spectranet customer portalEquipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. 99) (图2)。 光谱扫描结果显示在溶菌酶降解后其发射 光波长峰值从350nm迁移到355. Expanded wavelength coverage. The mineral-sorbed BG activity was measured by removing the supernatant and replacing it with buffer. Overview of the SpectraMax® i3x Multi-M. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. When the user applies a set temperature of 37°C, the top is heated to 37. baseada em NFC e fluxos de trabalho touchscreen aprimorados oferecem usabilidade de walk-up Os leitores de microplacas SpectraMax® iD3 e iD5 Multi-Mode medem absorbância, fluorescência e. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Figure 1. Check out our interactive. SpectraMax Luminometer Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. The current Firmware version is 1. Also note that these readers are not designed for extended shaking as this may damage the internal components. 100 µL of MycoAlert Substrate (reconstituted in MycoAlert Buffer) was immediately added to each well and the plate incubated at room temperature for 10 minutes. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 2 5039228D Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. SoftMax Pro GxP Compliance Software. Engineered for high performance, the auto dual injector cartridge includes fail safe features. 吸光、蛍光、発光測定に対応。. . Request a Quote!The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. AquaMax 2000 and 4000 Microplate Washers. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Innovative Solutions for Drug Discovery and Life Sciences Research 3 SpectraMax® iD3 Three-mode plate reader with large, high-res. Wait 20 seconds. Recommended settings for SpectraMax iD5 and i3x readers. 2. The Molecular Devices SpectraMax iD3 Multi-Mode Microplate Reader offers cutting-edge optical design and advanced detection technologies. A wide linear dynamic range ensures accurate assay results across a broad span of. All data are in μM and are n ≥ 3 independent experiments. Molecular Devices SpectraMax iD3 Installation Manual (26 pages) Multi-Mode Microplate Reader. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers merupakan alat yang dapat mengukur absorbansi, fluoresensi, dan pendaran. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. If no sound is heard during initialization, contact technical support. The•Spectramax id3 fluorescence plate reader •96 well plate •DH5 -mcherry w/ pMADRID ! NT for “non-targeting” •DH5 -mcherry w/ pMINT #2 ! G2 for “guide 2” •DH5 -mcherry w/ pMINT #3 ! G3 for “guide 3” •Wild type DH5 ! DH for “DH5 ” Protocols Measuring Fluorescence of Various E. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. 313 reference gain check fail. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Built-in near-field communication (NFC)SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 4 µL DNA standards. 315 can't find zero order. StackMax Microplate Handling System. ) Click the "OK" button on SoftMax Pro Setup dialog box . SpectraMax® iD3 SpectraMax® iD5 SpectraMax® i3x ABSFL LUMI CELL IMAGING WESTERN BLOT INJECTOR TRF FP ALPHA-SCREEN FRET ABS. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 7 mm for all shake intensities. See SpectraMax® Microplate Reader User Manual Download Page. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. GloMax Discover System. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Select the Driver tab and click Update Driver. 7°C and the bottom to 36. Unlock your i3/i3x instrument after shipment. It features a touchscreen interface, a built-in camera, and a patented optical system for optimal performance. Web fluorescence detection using the spectramax id5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the alexa594. SpectraMax iD3 Multi-Mode Microplate Reader. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. {"payload":{"allShortcutsEnabled":false,"fileTree":{"References_and_Manuals/SpectraMax_iD3_Manual":{"items":[{"name":". Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. SOD activity was. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 1. Step 4. The plates were then incubated at 37 °C for 10 min in the dark after the addition of 100 μL of 1 × JC-1 reagent to the wells. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. Application Area: Kinteic enzymatic assays, BCA Protein, HCP ELISA. SpectraMax® iD3 SpectraMax® iD5 SpectraMax® i3x ABSFL LUMI CELL IMAGING WESTERN BLOT INJECTOR TRF FP ALPHA-SCREEN FRET ABS. Additional information is also available in the User Manuals. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. The SpectraMax iD3 reader is fully compatible with the SpectraDrop™ Micro-Volume Microplate, enabling quantitation of precious low-volume samples. SpectraMax iD3 reader connection models. The SpectraMax i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6 - 384 well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. . The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. In addition, the iD5 reader measures TRF and FP. The latest news, articles, and resources, sent to your inbox weekly. Agonist EC 50 values obtained from Calcium 6-QF dye-loaded THP-1 cells. The SpectraMax MiniMax 300 Imaging Cytometer option allows for live cell images and analysis (below, left). Using the SpectraMax iD3 and iD5 readers with the SpectraMax Injector Cartridge with SmartInject Technology, and FLIPR Calcium 6 and 6-QF Assay Kits, we have demonstrated that it is possible to develop robust and reproducible kinetic assays. Compounds were tested for inhibition of 3CL pro following a similar protocol with several small modifications. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesSpectraMax® iD5 Five-mode plate reader including TRF, tunable FP, and western blot detection with large, high-res touchscreen, NFC functionality, ultra-cooled PMT and SmartInject™ Technology. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly match your needs. More than a playlist. These robust readers have been placed in labs from Antarctica to the. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for simplified data acquisition and analysis. . Videos & WebinarsThe SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. spr SpectraMax M2 M2e M3 M4 M5 M5e. Use the 2. The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 5nM并且 有很好的线性(r2>0. Built-in near-field communication (NFC. BMG Labtech - FLUOSTAR OMEGA. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Equipped with a high-performance, monochromator-based spectrophotometer, it offers flexible wavelength selection, allowing the user to conduct absorbance, fluorescence, and luminescence measurements with ease. 2. The chamber contains springs that position the cuvette in the proper alignment for a read. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Patented user-exchangeable cartridge design expands the system's detection capability making it. The company’s SoftMax® Pro Software is designed to achieve full compliance with FDA 21 CFR Part 11, with streamlined workflows to ensure data integrity. Unparalleled performance on a personalized platform. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. Description 1. Terms and Conditions Privacy Policy Online Terms of Use. Products : SpectraMax i3x reader, SoftMax Pro GxP Software, SpectraMax i3, SoftMax Pro Software, SpectraMax MiniMax cytometer. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. With optimized reagents and the industry-leading data acquisition. SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Standards were plotted using a log-log curve fit in SoftMax Pro Software. Every step is optimized to simplify analysis and reporting to support Molecular. Description 1. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Laboratory Equipment Molecular Devices ImageXpress Pico Installation Manual 82 pages. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. 4-µL DNA standards from 2 ng/µL to 1000 ng/µL were read in absorbance detection mode with a preconfigured protocol in SoftMax ProThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax MiniMax 300 Imaging Cytometer option allows for live cell images and analysis (below, left). The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectlyThe SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The SpectraMax® iD3 is a Multi-Mode Microplate Reader that offers the ideal solution for any application or budget. Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Category 2 and 3 microplate readers use gradient heating as well. Rating: 4. Prepare your iD series instrument for shipment. 6 (rated by 49 users) Compatible devices: FilterMax F3, SpectraMax MiniMax 300, SkanWasher 300, Digidata 1550, ClonePix 2, SpectraMax iD3, SpectraMax Paradigm, SpectraMax. With optimized reagents, option to operate the. spr SpectraMax iD5 SpectraTest ABS2 v1. 5054747F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 15 Optics 16 PlateControls 16Mitochondrial functions are frequently altered in cancer to promote both the energy and the necessary metabolic intermediates for biosynthesis required for tumor growth. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. Absorbance at 450 nm and 690 nm (as reference) was measured by using SpectraMax iD3 plate reader (Molecular Devices; San Jose, CA, USA). SpectraMax® iD3 多功能微孔读板机是一台能够完善实验解决方案的基础设备，能够帮助您提高研究能力。SoftMax® Pro 7 软件是一款业内认可的数据采集分析工具，其内置针对主流试剂优化的检测模板，通过自定义 SpectraMax iD3 的 工作流程来满足您的个性化需求。 主. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. The SpectraMax iD3 Microplate Reader is a versatile and reliable instrument that can measure absorbance, fluorescence, and luminescence in microplates. The SpectraMax iD3/iD5 readers use SoftMax ® Pro Data Acquisition & Analysis. Recent studies have shown that while non-stem, bulk cancer cells utilize glycolysis. Wait 20 seconds.